Stud Newsletter 2018

The team of dedicated stud vets at the Sussex Equine Hospital is made up of Ed Lyall, Paula Broadhurst, Simon Staempfli and Noelle Baxter. Together they provide a wide range of stud medicine services, including Artificial Insemination (AI) and Embryo Transfer (ET).  The team are all very experienced and hold postgraduate qualifications in reproductive medicine. Una Boyle, the fifth member of the team, joined the practice in 2016.

Sussex Equine Hospital is the only practice in the south of England that has a team of dedicated stud vets that provide routine and emergency services to stud clients 24 hours a day, 7 days a week for the entire stud season. This means that if your mare requires routine work to be done as part of her AI programme on a Sunday it will be a stud vet who does it, not just the duty vet. It also means that at night when your mare is foaling or your foal is not well, a stud vet with experience will be on hand to attend.

The hospital is fully equipped with a lab, foal care facilities and a surgical suite for performing all necessary mare and foal procedures including caesarean sections and assisted foaling under general anaesthesia.

Following is a brief overview of AI and ET; if you have any queries or want to discuss your requirements please do not hesitate to contact the practice and speak to one of the stud vet team.

Artificial Insemination

AI is the technique used to transfer appropriately processed semen, collected from a stallion, into the uterus of a mare at the correct time in her oestrus cycle in order to obtain a single pregnancy. The semen can either be fresh, chilled or frozen and it means stallions in the UK and abroad can be used, even semen from deceased stallions. Fresh semen is usually collected, extended and stored at room temperature in an airtight, light free container for use within 8 hours. Semen that is to be used longer than 8 hours after, but within 48 hours of collection should be chilled to 4ºC and stored for shipping in a special container. Semen that is required to last longer than 48 hours is frozen in liquid nitrogen at a temperature of -196ºC. The success of an AI programme is very dependent not only on the stallion’s semen but also on the careful veterinary management of the mare pre and post covering. This means that the semen is placed in the uterus at the correct stage in the mare’s cycle and that appropriate post insemination checks and treatments are made.

The other advantages of AI include keeping your mare and foal at home under your own supervision. Additionally, we find the intensive veterinary management required with mares to successfully perform AI can, in many circumstances, improve the chances of obtaining a pregnancy, as the probability of infection either bacterial or venereal is reduced. The addition of extenders and antibiotics to the semen can also improve the fertility of some stallions by improving the lifespan of the sperm. AI allows for the safe mating of mares or stallions with injuries and can prevent injury to valuable stallions by mares of poor temperament at natural mating.

At the Sussex Equine Hospital we use the most up-to-date techniques to manage AI mares, including insemination of frozen semen using a deep intrauterine technique. Over the last few years this technique has been used to inseminate mares with small volumes of chilled as well as frozen semen from the continent and we have seen a significant rise in the in-foal rate. Experience of dealing with thousands of mares over the years has allowed us to develop effective, simple routines and protocols for artificial insemination. Attention to detail is the key to good mare fertility and achieving a pregnancy as quickly as possible. The collection of frozen semen can allow a stallion to compete internationally without having to worry about stud duties and temperament changes whilst covering.

Embryo Transfer

Similarly Embryo Transfer (ET) is a technique that has been developed to enable mares to continue to compete while still producing foals. A mare’s fertility decreases as she gets older and this reduction in fertility appears to be more rapid in mares that have never had a foal. Therefore, once a mare is retired from competition in her teenage years and put to stud, her ability to become pregnant can actually be very poor.

To try and avoid this situation, owners will sometimes breed from fillies at 2 to 3 years of age prior to breaking in, so that they at least have 1 offspring before starting a competition career. Alternatively, ET can be used to produce foals while the mare is actively competing. Embryo Transfer involves the careful synchronisation of a donor mare (the mare you want the foal from) with a recipient mare (the mare we will transfer the pregnancy into). Then on a day individually selected depending on the situation, (usually day 7 or 8 after ovulation), the embryo is carefully flushed out of the donor mare and transferred to the recipient mare. A pregnancy scan is then performed about 1 week later on the recipient mare and subsequent scans as indicated.

The synchronisation of the recipient mare with the donor mare plays a large part in the success rate of this procedure and it can be quite difficult to do as not all mares ‘read the text book’ and respond to the medications the way we would like. Therefore, we ideally need to start off with a number of potential recipient mares so if some don’t respond as hoped we still have others to choose from. Fortunately, studies have shown no decrease in the success rate of the transfers, if the embryos are carefully chilled and transported within 24 hours to a facility with a number of recipient mares available.

Since 2008 the stud vet team at the Sussex Equine Hospital have performed a large number of successful ETs by flushing the donor mare and directly transferring the embryo into a recipient mare or sending the embryo chilled to a recipient mare facility. The donor mares are covered via AI in their own stable yard or at one of our AI centres. The flush can be carried out at the yard, AI centre or at our hospital. The flushing process takes between 30 and 60 minutes to perform, then the donor mare can go home to continue her normal competition routine. Once processed properly the embryo is either directly transferred into the recipient or transported chilled, same day by courier to the recipient mare facility where the embryo is transferred into the mare that was best synchronised with the donor.

Image shows a 7 day embryo

Over the last few years we have paid attention to detail and developed protocols that have given us excellent success rates with transferred embryos.

We aim to allow sport horse mares, that are of importance for breeding, to remain in work and competition. Our protocols mean that mares can remain at home with the rider so there is no check in training or competition.

New for 2018 we will be offering a service in the hospital where we will be able to freeze embryos, these can be stored for future transfer or even sold to other parties.

Packages for Veterinary Stud Work

Prior to embarking on an AI or ET programme with a mare it is important to become aware of all the facts and to balance out the pros and cons. One of the most important factors to consider is the cost. The keep, transport and veterinary costs sending the mare away to stud, must be balanced against the veterinary costs of AI or ET and keeping the mare at home. Many of the veterinary costs will be incurred either way and so it can be a real advantage to keep the mare at home. Some veterinary practices, as we do, will have a fixed price scheme for AI programmes. These can vary considerably, they may require the mare to go to a stud that the vet attends regularly and some may not include visit fees, pre-breeding swabs or pregnancy scans - when making price comparisons look very carefully at what is included in the package!

If you are interested in breeding from your mare in 2018 via natural cover, AI or ET contact the SEH now so that one of our dedicated Stud Vet team can explain the process in greater depth to you, one on one. Your options and which one of our competitive package deals best suits your individual situation can then be discussed. Mares can be worked on at owners’ yards within the practice area or mares from further afield can be boarded at the hospital or at one of several AI centres that we service.

Foal Care

Not only does the stud team take great care to get mares in foal as quickly as possible, they are also dedicated to the health of the newborn foal. We perform post foaling checks on both mares and foals within the first 12 hours after foaling, blood samples can be taken to assess health and also to make sure enough antibodies have passed from the mare to the foal by monitoring the IGG level of the foal's blood. All samples are processed in our own lab at the hospital.

The team will advise on future foal management, particularly of the limbs of the foal and also any other medical issues, as well as routine protocols such as worming and vaccination.

The hospital is equipped with two purpose built intensive care foal boxes, this allows us to deal with very sick foals and provide them with the very best levels of medicine possible. The hospital also has the surgical facilities required to perform all necessary mare and foal surgeries.

Stallion Semen Collection

Also new for the 2018 season we will be offering semen collection from stallions for fresh and chilled use. We will be able to send out semen 5 days a week across the UK. This service will be available to all stallion owners, however, prior to collection all stallions will need to have been tested to meet the strict health guidelines - for more information please do not hesitate to contact one of the stud team.